Methodological considerations for measuring spontaneous physical activity in rodents.
نویسندگان
چکیده
When exploring biological determinants of spontaneous physical activity (SPA), it is critical to consider whether methodological factors differentially affect rodents and the measured SPA. We determined whether acclimation time, sensory stimulation, vendor, or chamber size affected measures in rodents with varying propensity for SPA. We used principal component analysis to determine which SPA components (ambulatory and vertical counts, time in SPA, and distance traveled) best described the variability in SPA measurements. We compared radiotelemetry and infrared photobeams used to measure SPA and exploratory activity. Acclimation time, sensory stimulation, vendor, and chamber size independently influenced SPA, and the effect was moderated by the propensity for SPA. A 24-h acclimation period prior to SPA measurement was sufficient for habituation. Principal component analysis showed that ambulatory and vertical measurements of SPA describe different dimensions of the rodent's SPA behavior. Smaller testing chambers and a sensory attenuation cubicle around the chamber reduced SPA. SPA varies between rodents purchased from different vendors. Radiotelemetry and infrared photobeams differ in their sensitivity to detect phenotypic differences in SPA and exploratory activity. These data highlight methodological considerations in rodent SPA measurement and a need to standardize SPA methodology.
منابع مشابه
The Effect of physical Activity on Spontaneous Electroencephalographic Activity in Rat
Introduction:Exercise and physical activity are important factors for human health.It has been reported that exercise can be considerably useful in the teratment of psychological diseases.in the present study the effect of running on Spontaneous Electroencephalographic Activity (EEG) of rats was investigated. Method:Male wistar rats weighting 190-250 gr were selected and divided into contro...
متن کاملLong-term consequences of anterior cruciate ligament injury
.................................................................................................. 7 SVENSK SAMMANFATTNING ....................................................................... 9 ABBREVIATIONS ...................................................................................... 11 ORIGINAL PAPERS ...................................................................................
متن کاملA low-cost system to easily measure spontaneous physical activity in rodents.
Spontaneous physical activity (SPA) can be responsible for variations of a lot of physiological parameters at the molecular, cellular, tissue, and systemic levels. It is increasingly recognized that good understanding of a large part of experimental results requires weighting them by SPA in order to reduce variability and thus to decrease the number of animals necessary to conduct a study. Howe...
متن کاملResting-State Functional Connectivity in the Infant Brain: Methods, Pitfalls, and Potentiality
Early brain development is characterized by rapid growth and perpetual reconfiguration, driven by a dynamic milieu of heterogeneous processes. Postnatal brain plasticity is associated with increased vulnerability to environmental stimuli. However, little is known regarding the ontogeny and temporal manifestations of inter- and intra-regional functional connectivity that comprise functional brai...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- American journal of physiology. Regulatory, integrative and comparative physiology
دوره 306 10 شماره
صفحات -
تاریخ انتشار 2014